You can see a lot by observing: Optical Microscopy 2.0

View You can see a lot by observing: Optical Microscopy 2.0

Presenter: Steve Chu

Published: July 2014

Age: 18-22 and upwards

Views: 835 views

Tags: resolution;microscope;super;storm;palm

Type: Lectures

Source/institution: Lindau-Nobel

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Biological research and medicine were transformed by the invention and improvement of the optical microscope. Since the early 1990s, there has been another revolution in optical imaging, and manipulation of individual biological molecules and bio-molecular systems have been demonstrated and applied to a wide variety of systems. Most recently, innovations in ‘super-resolution’ optical imaging, such as STORM and PALM have been used to construct biological images with ~ 10 nm resolution. With bright optical probes and corrections to the slight differences in sensitivity to the CCD or CMOS camera, < 1 nm resolution is possible in biological samples in water. Recent applications of super-resolution imaging to cancer signaling, and bio-films will be discussed. Finally, the development of sub-wavelength micro-spectroscopy in the fingerprint region of the infrared spectra (wavelength = 4 - 12 ┬Ám) to observe changes in biological states with 20 nm spatial resolution will be outlined.